Abstract
Laser manipulation is widely used to study mechanics from the molecular to the tissue scale. We implemented optical tweezers to directly manipulate single cell-cell junctions in a developing tissue. We further extended the approach to two-point laser manipulation to enable extensive remodeling of cell-cell junctions. Here, we describe two-point laser manipulation and its implementation to probe the mechanics of cell junctions in the Drosophila embryo.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Nishizawa, K., Chardès, C., Clément, R., Lenne, PF. (2023). Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo. In: Zaidel-Bar, R. (eds) Mechanobiology. Methods in Molecular Biology, vol 2600. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2851-5_7
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DOI: https://doi.org/10.1007/978-1-0716-2851-5_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2850-8
Online ISBN: 978-1-0716-2851-5
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