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Generation of Insulin-Producing Multicellular Organoids

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2592))

Abstract

Clinical islet transplantation (CIT) is an established noninvasive treatment for type I diabetes (T1D) and has demonstrated improved glycemic control, preventing the occurrence of severe hypoglycemia. However, CIT has several limitations, such as the need for multiple donors, lifelong immunosuppression, and suboptimal long-term graft function. Most of the transplanted islets are lost due to inflammation, ischemic damage, and delayed revascularization.

Generation of organoids have gained increasing interest in regenerative medicine in recent years. In the context of beta-cell replacement, it offers a possibility to address limitations of CIT by allowing to produce uniform organoids from single or multiple cell types facilitating revascularization and anti-inflammatory and/or immunomodulatory protection. We have previously generated multicellular insulin-secreting organoids composed of islet cells and the human amniotic epithelial cells (hAECs). These 3D insulin-secreting structures demonstrated improved viability and function both in vitro and in vivo. Here we detail a stepwise methodology to generate insulin-secreting organoids using two different methods. In addition, quality assessment in vitro tests are also described.

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Author information

Authors and Affiliations

  1. Laboratory of Tissue Engineering and Organ Regeneration, Department of Surgery, University of Geneva, Geneva, Switzerland

    Laura Mar Fonseca, Fanny Lebreton & Ekaterine Berishvili

  2. Cell Isolation and Transplantation Center, Department of Surgery, Geneva University Hospitals and University of Geneva, Geneva, Switzerland

    Laura Mar Fonseca, Fanny Lebreton & Ekaterine Berishvili

  3. Faculty Diabetes Center, University of Geneva Medical Center, Geneva, Switzerland

    Laura Mar Fonseca, Fanny Lebreton & Ekaterine Berishvili

  4. Department of Visceral Surgery, Geneva University Hospitals, Geneva, Switzerland

    Charles-Henri Wassmer

  5. Institute of Medical and Public Health Research, Ilia State University, Tbilisi, Georgia

    Ekaterine Berishvili

Authors
  1. Laura Mar Fonseca
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  2. Fanny Lebreton
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  3. Charles-Henri Wassmer
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  4. Ekaterine Berishvili
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Corresponding author

Correspondence to Ekaterine Berishvili .

Editor information

Editors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Anna Moore

  2. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Ping Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fonseca, L.M., Lebreton, F., Wassmer, CH., Berishvili, E. (2023). Generation of Insulin-Producing Multicellular Organoids. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2807-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2806-5

  • Online ISBN: 978-1-0716-2807-2

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