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Optimal CCN4 Immunofluorescence for Tissue Microarray

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CCN Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2582))

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Abstract

CCN4 (also known as WNT1-Inducible Signaling Pathway Protein 1 or WISP1) is a 367 amino acid, 40 kDa protein located on chromosome 8q24.1-8q24.3. Prior studies have provided support for a pro-inflammatory role for CCN4. We have shown recently that CCN4 expression is associated with advanced disease, epithelial-mesenchymal transition, and an inflamed tumor microenvironment in multiple solid tumors. We detail here the CCN4 tissue microarray immunofluorescence protocol related to these findings.

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Acknowledgements

The development and optimization of this immunofluorescence protocol was supported by a Fonds de Recherche Québec–Santé’s (FRQS) Resident Physician Health Research Career Training Program (32667) to Pierre-Oliver Gaudreau and an operating grant to John Stagg from the Canadian Institutes of Health Research (project grant number: 364476). The work was also supported in part by a grant to M. Young from the National Institute of Dental and Craniofacial Research, NIH (grant number: Z01 DE000379-21).

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Authors and Affiliations

  1. Department of Oncology, Queen’s University and Canadian Cancer Trials Group, Kingston, ON, Canada

    Pierre-Olivier Gaudreau

  2. Centre de recherche du Centre hospitalier de l’Université de Montréal, Institut du Cancer de Montréal, Montréal, QC, Canada

    Isabelle Cousineau & John Stagg

  3. Faculté de Pharmacie, Université de Montréal, Montréal, QC, Canada

    John Stagg

Authors
  1. Pierre-Olivier Gaudreau
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  2. Isabelle Cousineau
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  3. John Stagg
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Corresponding author

Correspondence to Pierre-Olivier Gaudreau .

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Editors and Affiliations

  1. Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gaudreau, PO., Cousineau, I., Stagg, J. (2023). Optimal CCN4 Immunofluorescence for Tissue Microarray. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 2582. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2744-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2744-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2743-3

  • Online ISBN: 978-1-0716-2744-0

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