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Cardiac Gene Therapy

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Kiyotake Ishikawa

    1. Icahn School of Medicine at Mount Sinai, New York, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2573)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy

      • Francisco J. Romeo, Spyros A. Marvopoulos, Kiyotake Ishikawa
      Pages 3-10

  3. Gene Suppression, Editing, and Reprogramming

    1. Front Matter

      Pages 11-11
      Download chapter PDF

    2. Tough Decoy-Mediated Cardiac Gene Suppression

      • Changwon Kho
      Pages 13-30

    3. Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo

      • Camilla Paoletti, Carla Divieto, Valeria Chiono
      Pages 31-40Open Access

    4. CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC-Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis

      • Naohiro Yamaguchi, Xiao-Hua Zhang, Martin Morad
      Pages 41-52

    5. Enhancing Cardiomyocyte Transcription Using In Vivo CRISPR/Cas9 Systems

      • Eric Schoger, Laura C. Zelarayán
      Pages 53-61

    6. AAV-Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model

      • Tilman Ziegler, Tarik Bozoglu, Christian Kupatt
      Pages 63-74

  4. Cardiac Gene Therapy Vectors and Promotors

    1. Front Matter

      Pages 75-75
      Download chapter PDF

    2. Optimization of Synthesis of Modified mRNA

      • Jimeen Yoo, Lior Zangi
      Pages 77-87

    3. Design and Production of Heart Chamber-Specific AAV9 Vectors

      • Alina S. Bilal, Donna J. Thuerauf, Erik A. Blackwood, Christopher C. Glembotski
      Pages 89-113

    4. Generation of Atrial-Specific Construct Using Sarcolipin Promoter-Associated CRM4 Enhancer

      • Dongtak Jeong
      Pages 115-132

  5. Cardiac Gene Delivery Methods

    1. Front Matter

      Pages 133-133
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    2. Cardiac Targeted Adeno-Associated Virus Injection in Rats

      • Michael G. Katz, Yoav Hadas, Adam S. Vincek, Nataly Shtraizent, Eric Schadt, Efrat Eliyahu
      Pages 135-145

    3. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

      • Spyros A. Mavropoulos, Kelly P. Yamada, Tomoki Sakata, Kiyotake Ishikawa
      Pages 147-158

    4. Locked Nucleic Acid AntimiR Therapy for the Heart

      • Sabine Samolovac, Rabea Hinkel
      Pages 159-169

    5. Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection

      • Philipp Schlegel, Philip W. J. Raake
      Pages 171-177

    6. Endocardial Gene Delivery Using NOGA Catheter System

      • Satu Siimes, Niko Järveläinen, Henna Korpela, Seppo Ylä-Herttuala
      Pages 179-187

    7. Surgical Methods for Cardiac Gene Delivery in Large Animals

      • Michael G. Katz, Yoav Hadas, Adam S. Vincek, Nataly Shtraizent, Hylton P. Gordon, Peter Pastuszko et al.
      Pages 189-203
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Keywords

About this book

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. 

Chapter “Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.



Editors and Affiliations

  • Icahn School of Medicine at Mount Sinai, New York, USA

    Kiyotake Ishikawa

Bibliographic Information

  • Book Title: Cardiac Gene Therapy

  • Book Subtitle: Methods and Protocols

  • Editors: Kiyotake Ishikawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2707-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2706-8Published: 31 August 2022

  • Softcover ISBN: 978-1-0716-2709-9Published: 01 September 2023

  • eBook ISBN: 978-1-0716-2707-5Published: 30 August 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 347

  • Number of Illustrations: 9 b/w illustrations, 69 illustrations in colour

  • Topics: Genetics and Genomics, Cardiology, Human Genetics

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