Abstract
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in the histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix, cryoprotect, and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kiernan JA (2008) Histological and histochemical methods: theory and practice, Scion, Bloxham, Oxfordshire
Mills SE (2007) Histology for pathologists. Lippincott Williams & Wilkins, Philadelphia
Carriel V, Garzon I, Alaminos M, Campos A (2011) Evaluation of myelin sheath and collagen reorganization pattern in a model of peripheral nerve regeneration using an integrated histochemical approach. Histochem Cell Biol 136:709–717
Kiernan JA (2007) Histochemistry of staining methods for normal and degenerating myelin in the central and peripheral nervous systems. J Histotechnol 30:87–106
Kumar V, Abbas AK, Aster JC, Robbins SL (2013) Robbins basic pathology. Elsevier Saunders, Philadelphia
Carriel V, Garzon I, Alaminos M, Cornelissen M (2014) Histological assessment in peripheral nerve tissue engineering. Neural Regen Res 9:1657–1660
Cook DJ, Warren PJ (2015) Cellular pathology: introduction to techniques and applications, Scion, Bloxham, Oxfordshire
Burnett D, Crocker J (2005) The science of laboratory diagnosis. Wiley, Chichester
Di Scipio F, Raimondo S, Tos P, Geuna S (2008) A simple protocol for paraffin-embedded myelin sheath staining with osmium tetroxide for light microscope observation. Microsc Res Tech 71:497–502
García-García OD, Weiss T, Chato-Astrain J, Raimondo S, Carriel V (2022) Staining methods for normal and regenerative myelin in the nervous system. In: Histochemistry of single molecules: methods and protocols, 2nd edn. Methods Mol Biol 2566:187–203
Tracy RE, Walia P (2002) A method to fix lipids for staining fat embolism in paraffin sections. Histopathology 41:75–79
Tracy RE, Walia P (2004) Lipid fixation for fat staining in paraffin sections applied to lesions of atherosclerosis. Virchows Arch 445:22–26
Meier-Ruge WA, Bruder E (2005) Pathology of chronic constipation in pediatric and adult coloproctology. Pathobiology 72:1–102
Serrato D, Nieto-Aguilar R, Garzon I, Roda O, Campos A, Alaminos M (2009) Comparison of the effect of cryopreservation protocols on the histology of bioengineered tissues. Histol Histopathol 24:1531–1540
Weiss T, Taschner-Mandl S, Janker L, Bileck A, Rifatbegovic F, Kromp F et al (2021) Schwann cell plasticity regulates neuroblastic tumor cell differentiation via epidermal growth factor-like protein 8. Nat Commun 12:1624
Chato-Astrain J, García-García ÓD, Campos F, Sánchez-Porras D, Carriel V (2021) Basic nerve histology and histological analyses following peripheral nerve repair and regeneration. In: Phillips J, Hercher D, Hausner T (eds) Peripheral nerve tissue engineering and regeneration. Series in biomedical engineering. Springer, pp 1–37
Chato-Astrain J, Philips C, Campos F, Durand-Herrera D, Garcia-Garcia OD, Roosens A et al (2020) Detergent-based decellularized peripheral nerve allografts: an in vivo preclinical study in the rat sciatic nerve injury model. J Tissue Eng Regen Med 14:789–806
El Soury M, Garcia-Garcia OD, Moretti M, Perroteau I, Raimondo S, Lovati AB et al (2021) Comparison of Decellularization protocols to generate peripheral nerve grafts: a study on rat sciatic nerves. Int J Mol Sci 22:2389
Garcia-Garcia OD, El Soury M, Gonzalez-Quevedo D, Sanchez-Porras D, Chato-Astrain J, Campos F et al (2021) Histological, biomechanical, and biological properties of Genipin-Crosslinked Decellularized peripheral nerves. Int J Mol Sci 22:674
Philips C, Campos F, Roosens A, Sanchez-Quevedo MDC, Declercq H, Carriel V (2018) Qualitative and quantitative evaluation of a novel detergent-based method for decellularization of peripheral nerves. Ann Biomed Eng 46:1921–1937
Durand-Herrera D, Campos F, Jaimes-Parra BD, Sanchez-Lopez JD, Fernandez-Valades R, Alaminos M et al (2018) Wharton's jelly-derived mesenchymal cells as a new source for the generation of microtissues for tissue engineering applications. Histochem Cell Biol 150:379–393
Sanchez-Porras D, Durand-Herrera D, Paes AB, Chato-Astrain J, Verplancke R, Vanfleteren J et al (2021) Ex vivo generation and characterization of human hyaline and elastic cartilaginous microtissues for tissue engineering applications. Biomedicine 9:292
Acknowledgments
Procedures described in this chapter were supported by the Spanish “Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III); Grants Nos. FIS PI17-0393, FIS PI20-0318 co-financed by the “Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union”; Grant No. P18-RT-5059 by “Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020), Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía, España”; and Grant No. A-CTS-498-UGR18 by “Programa Operativo FEDER Andalucía 2014–2020, Universidad de Granada, Junta de Andalucía, España”, co-funded by ERDFFEDER, the European Union. The authors are grateful to Prof. Ariane Ruyffelaert for proofreading.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Sánchez-Porras, D., Bermejo-Casares, F., Carmona, R., Weiss, T., Campos, F., Carriel, V. (2023). Tissue Fixation and Processing for the Histological Identification of Lipids. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_14
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2675-7_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2674-0
Online ISBN: 978-1-0716-2675-7
eBook Packages: Springer Protocols