Abstract
DNA-encoded library (DEL) links the powers of genetics and chemicals via high-efficient enzymatic ligation of DNA barcodes and the “split and pool” combinatorial synthesis. Natural products (NPs) are evolutionary optimized compounds that have played a key role in the history of human drug discovery. Herein, we describe a method for functionality-independent annotation of complex natural products with amplifiable DNA barcodes to generate a DNA-encoded natural product library (nDEL). This method provides a simple and practical solution to leverage natural products by DNA barcoding.
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Acknowledgment
We acknowledge research funding from the National Natural Science Foundation of China (Grants 21502114, 31770776, and 31500632) and the Science and Technology Commission of Shanghai Municipality (Grant 16DZ1910200).
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Xu, H., Ma, P. (2022). DNA Encoding of Natural Products. In: Israel, D., Ding, Y. (eds) DNA-Encoded Chemical Libraries. Methods in Molecular Biology, vol 2541. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2545-3_13
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DOI: https://doi.org/10.1007/978-1-0716-2545-3_13
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