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Functional Annotation of miRNAs in Rice Using ARMOUR

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Genomics of Cereal Crops

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Abstract

The role of miRNAs and significance of their interaction with the mRNAs has been well established in a wide range of essential biological processes in plants. Many online databases are available for reporting the miRNAs and their target transcripts in a variety of plants. ARMOUR (ARice miRNA–mRNA interaction resource) presents a cohesive database for all analysis related to miRNAs and their predicted target mRNAs across 7 Indian rice cultivars in 38 different tissue or abiotic stress conditions. It covers profiles of 689 known and 1664 putative novel miRNAs. The information on miRNA profiles is supplemented by the sequence information of mature and hairpin structures. ARMOUR provides the flexibility to query the database in multiple ways using preset or custom text searches. It also facilitates searching for the target mRNAs, determining the gene ontology (enrichment and their associated biological pathways. The interactive user interface allows ARMOUR to serve as an integrated resource for investigation of miRNAs in rice and related plant species.

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Acknowledgments

The authors thank Dr. Deepti Mittal, Dr. Mohammed Aslam, and Dr. Neha Sharma for their help with library preparation. We are grateful to Ms. Rashmi Renu Sahoo, Mr. Yusuf Khan, and Ms. Anita Tripathi for assistance with analyzing the sequencing data. The authors thank team Bionivid for their help on developing the database. We acknowledge the help of Mr. Dario Palmisano in hosting the database on the website. The work was supported by grants from ICGEB and Department of Biotechnology, Government of India.

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Correspondence to Neeti Sanan-Mishra .

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Sanan-Mishra, N., Goswami, K. (2022). Functional Annotation of miRNAs in Rice Using ARMOUR. In: Wani, S.H., Kumar, A. (eds) Genomics of Cereal Crops. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2533-0_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2533-0_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2532-3

  • Online ISBN: 978-1-0716-2533-0

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