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Study of Amyloid Fibers Using Atomic Force Microscopy

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Bacterial Amyloids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2538))

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Abstract

Atomic force microscopy (AFM) provides high-resolution images of the topography of amyloid fibers adsorbed on surfaces. This information is very useful to study their molecular assembly under various conditions. This chapter describes the basic protocols required to deposit fibers on flat surfaces and discusses some of the practical issues required to operate a good commercial microscope setup to obtain appropriate high-resolution AFM topographic images of amyloid fibers.

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Author information

Authors and Affiliations

  1. Instituto de Catálisis y Petroleoquímica (CSIC), (Cantoblanco) Madrid, Spain

    Daniel G. Cava & Marisela Vélez

Authors
  1. Daniel G. Cava
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  2. Marisela Vélez
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Corresponding author

Correspondence to Marisela Vélez .

Editor information

Editors and Affiliations

  1. CEA Saclay, Lab Léon Brillouin UMR12 CEA/CNRS, Gif sur Yvette Cedex, France

    Véronique Arluison

  2. Synchrotron SOLEIL, Gif-sur-Yvette Cedex, France

    Frank Wien

  3. Departamento de Biología, University of Chile, Santiago, Chile

    Andrés Marcoleta

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cava, D.G., Vélez, M. (2022). Study of Amyloid Fibers Using Atomic Force Microscopy. In: Arluison, V., Wien, F., Marcoleta, A. (eds) Bacterial Amyloids. Methods in Molecular Biology, vol 2538. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2529-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2529-3_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2528-6

  • Online ISBN: 978-1-0716-2529-3

  • eBook Packages: Springer Protocols

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