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Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback

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Plant Pathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2536))

Abstract

The inoculum of H. fraxineus consists mainly of ascospores released from apothecia which are growing on fallen leaves infected during the previous year. The ascospores can be detected in various manners due to their high concentration in the air during the main sporulation season, which corresponds to astronomic summer. This methodology is focused on one of the methods which have been successfully used. It employs a cheap, but highly efficient rotating arm air sampler and a specific quantitative real-time PCR method for the quantification of the air samples. The methodology is accompanied by lots of detailed theoretical and practical notes for its smooth application, including mentioning other alternatives.

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Acknowledgment

This work was supported by using the laboratories of Mendel University in Brno, Phytophthora Research Centre (funded by project CZ.02.1.01/0.0/0.0/15_003/0000453), and laboratories of the University of South Bohemia in České Budějovice, Faculty of Agriculture and Technologies, Department of Genetics and Agricultural Biotechnology. Furthermore, this work was financed from a HORIZON 2020 project (agreement no. 771271): Holistic Management of Emerging Forest Pests and Diseases (HOMED).

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Authors and Affiliations

  1. Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Brno, Czech Republic

    Miloň Dvořák

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  1. Miloň Dvořák
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Correspondence to Miloň Dvořák .

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Editors and Affiliations

  1. Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Sesto Fiorentino, Florence, Italy

    Nicola Luchi

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Dvořák, M. (2022). Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2517-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2517-0_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2516-3

  • Online ISBN: 978-1-0716-2517-0

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