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Determination of Antibiotic Resistance Gene Transfer

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Biosafety Assessment of Probiotic Potential

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

Probiotics are recognized as safe by the US Food and Drug Administration (USFDA) and the European Food Safety. But many probiotic bacteria serve as a reservoir of antibiotic resistance genes. Antibiotic resistance (ABR) in bacteria is recognized worldwide as an important threat to humans and animal health, and it is imperatively essential to know the mechanisms of antibiotic resistance together with its emergence and dissemination. An important way of transmission of antibiotic-resistant bacteria in humans and animals occurs through food especially consumption of fermented food containing live probiotic bacteria which serve as a vehicle for antibiotic resistance, with a direct link between the animal indigenous microflora and the human gastrointestinal tract (GIT). Therefore, the safety evaluation of probiotics for antibiotic resistance gene transfer has been assessed by conjugation filter mating assay.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Government Arts and Science College (Women), Perambalur, Tamil Nadu, India

    K. R. Jeya

  2. Department of Biological Sciences, College of Sciences, King Faisal University, Al-Ahsa, Saudi Arabia

    Ashraf Khalifa

  3. Botany and Microbiology Department, Faculty of Sciences, Beni-Suef University, Beni-Suef, Egypt

    Ashraf Khalifa

  4. Department of Microbiology, Adhiparasakthi College of Arts and Science (Autonomous), Kalavai, Tamil Nadu, India

    M. Veerapagu

  5. Department of Life Sciences, Sri Sathya Sai University for Human Excellence, Kalaburagi, Karnataka, India

    A. Sankaranarayanan

Authors
  1. K. R. Jeya
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  2. Ashraf Khalifa
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  3. M. Veerapagu
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  4. A. Sankaranarayanan
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Editor information

Editors and Affiliations

  1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Mitesh Kumar Dwivedi

  2. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Natarajan Amaresan

  3. Department of Life Sciences, Sri Sathya Sai University for Human Excellence, Kalaburagi, Karnataka, India

    A. Sankaranarayanan

  4. Department of Biochemistry, The Maharaja Sayajirao University of Baroda, Vadodara, India

    Rasheedunnisa Begum

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Jeya, K.R., Khalifa, A., Veerapagu, M., Sankaranarayanan, A. (2022). Determination of Antibiotic Resistance Gene Transfer. In: Dwivedi, M.K., Amaresan, N., Sankaranarayanan, A., Begum, R. (eds) Biosafety Assessment of Probiotic Potential. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2509-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2509-5_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2508-8

  • Online ISBN: 978-1-0716-2509-5

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