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BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2525))

Abstract

By virtue of its high sensitivity, bioluminescence imaging (BLI) is an important tool for biosensing and bioimaging in life sciences. Compared to fluorescence imaging (FLI), BLI has a superior advantage that the background signals resulting from autofluorescence are almost zero due to the unnecessity of external excitation. In addition, BLI can permit a long-term observation of living cells because BL results in very low photocytotoxicity toward the host cells. Although BLI has such superior properties over FLI, the available wavelengths in BLI are mostly limited to the visible region. Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a quantum dot (QD) and luciferase–protein conjugate.

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Acknowledgments

This work is partly supported by the Ministry of Education, Science, Sport, and Culture of Japan (Grant-in-Aid for Scientific Research (B) 19H04459 to TJ).

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Authors and Affiliations

  1. RIKEN Center for Biosystems Dynamics Research, Suita, Osaka, Japan

    Setsuko Tsuboi & Takashi Jin

Authors
  1. Setsuko Tsuboi
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  2. Takashi Jin
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Corresponding author

Correspondence to Takashi Jin .

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Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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Tsuboi, S., Jin, T. (2022). BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2525. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2473-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2473-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2472-2

  • Online ISBN: 978-1-0716-2473-9

  • eBook Packages: Springer Protocols

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