Abstract
Various electron microscopy techniques were applied recently to the study of DNA condensation in dormant bacterial cells. Here, we describe, in detail, the preparation of dormant Escherichia coli cells for electron microscopy studies and electron tomography and energy dispersive spectroscopy (EDS) approaches, which were used to reveal the structures of DNA-protein complexes in dormant Escherichia coli cells.
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Acknowledgments
Bacterial strain with Dps overexpression was kindly provided by Prof. Vassili N. Lasarev, Head of the Laboratory of Gene Engineering, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russia. We thank Yana Danilova for her help with EM analysis and Lisa Trifonova for proofreading the manuscript.
The electron microscopy of DNA-protein complexes was performed on the Unique equipment setup “3D-EMС” of Moscow State University (under support from Russian Science Foundation, grant #19-74-30003). O.S.S. acknowledges the partial support from the Interdisciplinary Scientific and Educational School of Moscow Lomonosov University «Molecular Technologies of the Living Systems and Synthetic Biology».
Y.F.K. and A.M. are grateful for the support from The Ministry of Science and Higher Education of the Russian Federation (state assignments 122040400089-6), N.L. for the support from The Ministry of Science and Higher Education of the Russian Federation (state assignments 122040800164-6).
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Moiseenko, A., Loiko, N., Sokolova, O.S., Krupyanskii, Y.F. (2022). Application of Special Electron Microscopy Techniques to the Study of DNA – Protein Complexes in E. coli Cells. In: Peeters, E., Bervoets, I. (eds) Prokaryotic Gene Regulation. Methods in Molecular Biology, vol 2516. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2413-5_9
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DOI: https://doi.org/10.1007/978-1-0716-2413-5_9
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