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Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells

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Neuronal Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2515))

Abstract

The Nobel Prize–winning discovery that human somatic cells can be readily reprogrammed into pluripotent cells has revolutionized our potential to understand the human brain. The rapid technological progression of this field has made it possible to easily obtain human neural cells and even intact tissues, offering invaluable resources to model human brain development. In this chapter, we present a brief history of hPSC-based approaches to study brain development and then, provide new insights into neurological diseases, focusing on those driven by aberrant cell death. Furthermore, we will shed light on the latest technologies and highlight the methods that researchers can use to employ established hPSC approaches in their research. Our intention is to demonstrate that hPSC-based modeling is a technical approach accessible to all researchers who seek a deeper understanding of the human brain.

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Acknowledgments

This work was jointly supported by a Tier II Canada Research Chair award, start-up grants by the Faculty of Science and Department of Biological Sciences at Simon Fraser University, and grants awarded from the Cancer Research Society (Scholarships for the Next Generation of Scientists) and Natural Sciences and Engineering Research Council of Canada to L.M. Julian.

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  1. Department of Biological Sciences; Centre for Cell Biology, Development, and Disease, Faculty of Science, Simon Fraser University, Burnaby, BC, Canada

    George E. Allen, Aaron S. Dhanda & Lisa M. Julian

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  1. George E. Allen
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  2. Aaron S. Dhanda
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  3. Lisa M. Julian
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Correspondence to Lisa M. Julian .

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  1. Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Arezu Jahani-Asl

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Allen, G.E., Dhanda, A.S., Julian, L.M. (2022). Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells. In: Jahani-Asl, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 2515. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2409-8_20

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