Abstract
This chapter describes a simple and easy-to-do protocol to assay the level of lipid peroxidation in biological samples by a colorimetric method. The estimate of malonaldehyde, a secondary product of lipid oxidation, can be carried out by the TBARS assay, which measures the amount of thiobarbituric acid reactive substances forming a red complex with maximum absorbance at 532 nm against a calibration curve built with malonaldehyde.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Seyedsadjadi N, Grant R (2020) The potential benefit of monitoring oxidative stress and inflammation in the prevention of non-communicable diseases (NCDs). Antioxidants (Basel) 10:15. https://doi.org/10.3390/antiox10010015
Nediani C, Giovannelli L (2020) Oxidative stress and inflammation as targets for novel preventive and therapeutic approaches in non-communicable diseases. Antioxidants (Basel) 9:290. https://doi.org/10.3390/antiox9040290
Pisoschi AM, Pop A, Iordache F, Stanca L, Predoi G, Serban AI (2021) Oxidative stress mitigation by antioxidants – an overview on their chemistry and influences on health status. Eur J Med Chem 209:112891. https://doi.org/10.1016/j.ejmech.2020.112891
Del Rio D, Stewart AJ, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 15:316–328. https://doi.org/10.1016/j.numecd.2005.05.003
Mariutti LRB, Bragagnolo N (2015) Analysis methods for thiobarbituric acid reactive substances and malonaldehyde in food and biological samples. In: Advances in chemistry research, vol 29, 1st edn. Nova Science Publishers, New York, pp 100–130
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. https://doi.org/10.1016/0003-2697(79)90738-3
Cazarin CBB, Silva JK, Colomeu TC et al (2015) Intake of Passiflora edulis leaf extract improves antioxidant and anti-inflammatory status in rats with 2,4,6-trinitrobenzenesulphonic acid induced colitis. J Funct Foods 17:575–586. https://doi.org/10.1016/j.jff.2015.05.034
Batista AG, Lenquiste SA, Cazarin CBB et al (2014) Intake of jaboticaba peel attenuates oxidative stress in tissues and reduces circulating saturated lipids of rats with high-fat diet-induced obesity. J Funct Foods 6:450–461. https://doi.org/10.1016/j.jff.2013.11.011
De Leon JAD, Borges CR (2020) Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. J Vis Exp 159:e61122. https://doi.org/10.3791/61122
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this chapter
Cite this chapter
Mariutti, L.R.B. (2022). Lipid Peroxidation (TBARS) in Biological Samples. In: Betim Cazarin, C.B. (eds) Basic Protocols in Foods and Nutrition. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2345-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2345-9_7
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2344-2
Online ISBN: 978-1-0716-2345-9
eBook Packages: Springer Protocols