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Muscle and Fat Biopsy and Metabolomics

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Basic Protocols in Foods and Nutrition

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

The analysis of biological fluids and tissues in humans using a metabolomic approach can be somewhat complex if there is not an adequate collection and efficient sample preparation. The adjustment of spectrum acquisition parameters and data analysis are essential both in nuclear magnetic resonance spectroscopy (NMR) and in mass spectrometry (MS). This chapter will focus mainly on the methods and protocols for collecting and preparing biological fluids (blood, saliva, urine) and tissues (adipose and muscle) in humans using 1H NMR.

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Author information

Authors and Affiliations

  1. Exercise Physiology Laboratory (FISEX), School of Physical Education, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Cláudia Regina Cavaglieri, Mara Patrícia Traina Chacon-Mikahil, Renata Garbellini Duft, Ivan Luiz Padilha Bonfante, Arthur Fernandes Gáspari & Alex Castro

  2. Federal Institute of Education, Science and Technology of São Paulo - IFSP, Hortolândia, São Paulo, Brazil

    Ivan Luiz Padilha Bonfante

  3. Brazilian Sport Climbing Association, São Paulo, Brazil

    Arthur Fernandes Gáspari

  4. Sidia Institute of Science and Technology, Manaus, Amazonas, Brazil

    Arthur Fernandes Gáspari

  5. Nuclear Magnetic Resonance Laboratory, Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, São Paulo, Brazil

    Alex Castro

Authors
  1. Cláudia Regina Cavaglieri
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  2. Mara Patrícia Traina Chacon-Mikahil
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  3. Renata Garbellini Duft
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  4. Ivan Luiz Padilha Bonfante
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  5. Arthur Fernandes Gáspari
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  6. Alex Castro
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Corresponding author

Correspondence to Cláudia Regina Cavaglieri .

Editor information

Editors and Affiliations

  1. Faculty of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil

    Cinthia Baú Betim Cazarin

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Cavaglieri, C.R., Chacon-Mikahil, M.P.T., Duft, R.G., Bonfante, I.L.P., Gáspari, A.F., Castro, A. (2022). Muscle and Fat Biopsy and Metabolomics. In: Betim Cazarin, C.B. (eds) Basic Protocols in Foods and Nutrition. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2345-9_22

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  • DOI: https://doi.org/10.1007/978-1-0716-2345-9_22

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  • Publisher Name: Humana, New York, NY

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