Abstract
The production of recombinant proteins in bacteria made possible to obtain large quantities of proteins essential for basic and applied research. Escherichia coli remains one of the organisms of choice for recombinant proteins because of its ability to grow at high density and availability of a vast catalog of cloning vectors and mutant host strains. Here, we describe the protocols for the expression of cold-adapted (hemo)globins in Escherichia coli.
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Acknowledgments
This research was financially supported by the Italian National Programme for Antarctic Research (PNRA) of the Ministry of University and Research (MUR). We wish to thank Valentina Brasiello and Chiara Nobile for technical support and assistance.
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Giordano, D., Verde, C. (2022). Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_14
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DOI: https://doi.org/10.1007/978-1-0716-2313-8_14
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