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Applications of Genome Modulation and Editing

  • Book
  • © 2022

Overview

Editors:
  1. Paul John Verma

    1. Aquatics & Livestock Sciences, South Australian Research and Development Institute, Roseworthy, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Huseyin Sumer

    1. Faculty of Science, Engg & Tech, Swinburne University of Technology, Hawthorn, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Jun Liu

    1. Dept of Jobs, Precincts and Regions, Agriculture Victoria Research, Bundoora, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2495)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
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  2. Gene Editing Approaches

    1. Front Matter

      Pages 1-1
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    2. Historical DNA Manipulation Overview

      • Lluis Montoliu
      Pages 3-28

    3. Tools for Efficient Genome Editing; ZFN, TALEN, and CRISPR

      • Yasaman Shamshirgaran, Jun Liu, Huseyin Sumer, Paul J. Verma, Amir Taheri-Ghahfarokhi
      Pages 29-46

  3. Genetic Manipulation Methods

    1. Front Matter

      Pages 47-47
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    2. Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the piggyBac Transposon System

      • Jinchao Gu, Huseyin Sumer, Brett Cromer
      Pages 49-66

    3. Modifying Bacterial Artificial Chromosomes for Extended Genome Modification

      • Hannah Auch, Nikolai Klymiuk, Petra Runa-Vochozkova
      Pages 67-90

    4. Immortalised Cas9-expressing Cell lines for Gene interrogation

      • Luis F. Malaver-Ortega, Joseph Rosenbluh
      Pages 91-97

    5. Targeting the AAVS1 Site by CRISPR/Cas9 with an Inducible Transgene Cassette for the Neuronal Differentiation of Human Pluripotent Stem Cells

      • Jinchao Gu, Ben Rollo, Huseyin Sumer, Brett Cromer
      Pages 99-114

    6. Microinjection of Zygotes for CRISPR/Cas9-Mediated Insertion of Transgenes into the Murine Rosa26 Safe Harbor

      • Fabien Delerue, Lars M. Ittner
      Pages 115-128

    7. CRISPR-on for Endogenous Activation of SMARCA4 Expression in Bovine Embryos

      • Virgilia Alberio, Virginia Savy, Daniel F. Salamone
      Pages 129-148

  4. Applications of Genome Manipulation

    1. Front Matter

      Pages 149-149
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    2. CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique

      • Amanda Choo, Elisabeth Fung, Thu N. M. Nguyen, Anzu Okada, Peter Crisp
      Pages 151-171

    3. CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly

      • Daniel F. Paulo, Megan E. Williamson, Maxwell J. Scott
      Pages 173-201

    4. Generation of Gene Drive Mice for Invasive Pest Population Suppression

      • Mark D. Bunting, Chandran Pfitzner, Luke Gierus, Melissa White, Sandra Piltz, Paul Q. Thomas
      Pages 203-230

  5. Large Animal Models of Human Disease

    1. Front Matter

      Pages 231-231
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    2. Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System

      • Martina Crispo, Vanessa Chenouard, Pedro dos Santos-Neto, Laurent Tesson, Marcela Souza-Neves, Jean-Marie Heslan et al.
      Pages 233-244

    3. Targeted Gene Editing in Porcine Germ Cells

      • Taylor Goldsmith, Alla Bondareva, Dennis Webster, Anna Laura Voigt, Lin Su, Daniel F. Carlson et al.
      Pages 245-258

    4. Generating a Heat-Tolerance Mouse Model

      • Jun Liu, Paul J. Verma
      Pages 259-272

  6. Large Animal Welfare and Production Outcomes

    1. Front Matter

      Pages 273-273
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Keywords

About this book

This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols.

Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.

Editors and Affiliations

  • Aquatics & Livestock Sciences, South Australian Research and Development Institute, Roseworthy, Australia

    Paul John Verma

  • Faculty of Science, Engg & Tech, Swinburne University of Technology, Hawthorn, Australia

    Huseyin Sumer

  • Dept of Jobs, Precincts and Regions, Agriculture Victoria Research, Bundoora, Australia

    Jun Liu

Bibliographic Information

  • Book Title: Applications of Genome Modulation and Editing

  • Editors: Paul John Verma, Huseyin Sumer, Jun Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2301-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2300-8Published: 14 June 2022

  • Softcover ISBN: 978-1-0716-2303-9Published: 14 June 2023

  • eBook ISBN: 978-1-0716-2301-5Published: 13 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 369

  • Number of Illustrations: 15 b/w illustrations, 44 illustrations in colour

  • Topics: Genetics and Genomics

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