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Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2485))

Abstract

Neonatal mice display a remarkable ability to regenerate their heart following an injury during the first week of life. A key facet of successful cardiac regeneration is the proliferation of cardiomyocytes to replace the lost cells. Stimulating cardiomyocyte proliferation in the adult heart is a very promising approach to restore cardiac structure and function following injury. Here, we outline our approach to assess cardiomyocyte proliferation following a myocardial injury via the cell cycle markers phospho-histone H3 and Aurora B. We additionally discuss how we assess successful regeneration using wheat germ agglutinin to measure cardiomyocyte size, nuclear staining to quantify cardiomyocyte nucleation, and Trichrome staining to identify myocardial regeneration and scarring in the myocardium.

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Authors and Affiliations

  1. Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA

    Emma B. Brandt & Ahmed I. Mahmoud

  2. University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA

    Ahmed I. Mahmoud

Authors
  1. Emma B. Brandt
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  2. Ahmed I. Mahmoud
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Corresponding author

Correspondence to Ahmed I. Mahmoud .

Editor information

Editors and Affiliations

  1. School of Engineering, Brown University, Providence, RI, USA

    Kareen L.K. Coulombe

  2. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Lauren D. Black III

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Brandt, E.B., Mahmoud, A.I. (2022). Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2261-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2260-5

  • Online ISBN: 978-1-0716-2261-2

  • eBook Packages: Springer Protocols

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