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Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells

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Recombinant Proteins in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2480))

Abstract

This protocol describes a robust method to obtain transgenic Nicotiana tabacum BY-2 cells that produce glycoproteins of interest via Agrobacterium tumefaciens transformation. Compared to biolistics-based transformation, this procedure requires only standard laboratory equipment.

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Acknowledgments

We are especially grateful to Professor Marc Boutry for his constant interest in our research as well as his critical reading of the manuscript.

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Authors and Affiliations

  1. Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium

    Catherine Navarre & François Chaumont

Authors
  1. Catherine Navarre
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  2. François Chaumont
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Corresponding author

Correspondence to François Chaumont .

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Editors and Affiliations

  1. Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany

    Stefan Schillberg

  2. Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany

    Holger Spiegel

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Navarre, C., Chaumont, F. (2022). Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells. In: Schillberg, S., Spiegel, H. (eds) Recombinant Proteins in Plants. Methods in Molecular Biology, vol 2480. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2241-4_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2241-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2240-7

  • Online ISBN: 978-1-0716-2241-4

  • eBook Packages: Springer Protocols

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