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Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2437))

Abstract

Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

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Acknowledgments

R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick’s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558.

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Authors and Affiliations

  1. Department of Neuroscience, University of Kentucky College of Medicine, Lexington, KY, USA

    Tara R. Hawkinson & Ramon C. Sun

  2. Markey Cancer Center, Lexington, KY, USA

    Ramon C. Sun

Authors
  1. Tara R. Hawkinson
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  2. Ramon C. Sun
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Correspondence to Ramon C. Sun .

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Editors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, USA

    Young-Jin Lee

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Hawkinson, T.R., Sun, R.C. (2022). Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2030-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2029-8

  • Online ISBN: 978-1-0716-2030-4

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