Abstract
The filamentous cytoskeletal protein microtubule, a polymer of α and β heterodimers of tubulin, plays major roles in intracellular transport as well as in vitro molecular actuation and transportation. Functionalization of tubulin dimers through covalent linkage facilitates utilization of microtubule in the nanobioengineering. Here we present a detailed description of the methodologies used to modify tubulin dimers with DNA strand and biotin through covalent interaction.
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Acknowledgments
This work was financially supported by Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Engine” (JSPS KAKENHI Grant Number JP18H05423), and Grant-in-Aid for Scientific Research (A) (18H03673) from Japan Society for the Pro-motion of Science (JSPS).
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Akter, M., Keya, J.J., Kabir, A.M.R., Rashid, M.R., Ishii, S., Kakugo, A. (2022). Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA. In: Inaba, H. (eds) Microtubules. Methods in Molecular Biology, vol 2430. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1983-4_3
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DOI: https://doi.org/10.1007/978-1-0716-1983-4_3
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