Abstract
Surface ultrastructures of giant cells (GCs) may help distinguish an aggressive tumor from an indolent giant cell tumor (GCT). This protocol describes a better way for ultrastructural surface imaging of GC from GCT of bone by scanning electron microscope (SEM). Fresh GCT samples collected in Dulbecco’s modified Eagle medium (DMEM) are thoroughly washed to remove the blood and treated with collagenase to isolate the GCs. The collagenase-treated and critical point dried (CPD) samples yield a greater number of isolated GCs with better surface morphology, including membrane folding and micro-vesicular structures on the surface. Collagenase digestion and CPD should be performed for ultrastructural surface imaging of individual giant cells.
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Acknowledgments
The authors acknowledge the individual financial support given by All India Institute of Medical Sciences (AIIMS), New Delhi, as intramural grants to SCY (F.8-419/A-419/2016/RS) and ARM (F.8-594/A-594/2018/RS).
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Mridha, A.R., Yadav, S.C. (2022). Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone. In: Deep, G. (eds) Cancer Biomarkers. Methods in Molecular Biology, vol 2413. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1896-7_19
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DOI: https://doi.org/10.1007/978-1-0716-1896-7_19
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1896-7
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