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Vaccine Design pp 463–480Cite as

In Silico Design of Recombinant Chimera T Cell Peptide Epitope Vaccines for Visceral Leishmaniasis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2410))

Abstract

Visceral leishmaniasis (VL) is a neglected tropical disease caused by protozoan parasites of the genus Leishmania. Systemic VL is fatal if untreated and there are no prophylactic human vaccines available. Several studies suggest that Th1 cell-mediated immunity plays a major role in protecting against VL. In this chapter we describe a method for designing recombinant chimera vaccines in silico based on the prediction of T cell epitopes within protein antigens identified as potential protective immunogens. Development of a recombinant chimera protein (RCP) vaccine using T cell epitope peptides identified from four Leishmania proteins is used as an exemplar of this method.

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Acknowledgements

VL vaccine research is supported by grant MR/R005850/1 from the Medical Research Council (VAccine deveLopment for complex Intracellular neglecteD pAThogEns—VALIDATE), UK, and grant APQ-408675/2018-7 from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Amanda Sanchez Machado, Vivian Tamietti Martins & Eduardo Antonio Ferraz Coelho

  2. Neisseria Research Group, Molecular Microbiology, School of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, England, UK

    Maria Victoria Humbert & Myron Christodoulides

Authors
  1. Amanda Sanchez Machado
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  2. Vivian Tamietti Martins
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  3. Maria Victoria Humbert
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  4. Myron Christodoulides
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  5. Eduardo Antonio Ferraz Coelho
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Corresponding author

Correspondence to Myron Christodoulides .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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Machado, A.S., Martins, V.T., Humbert, M.V., Christodoulides, M., Coelho, E.A.F. (2022). In Silico Design of Recombinant Chimera T Cell Peptide Epitope Vaccines for Visceral Leishmaniasis . In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2410. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1884-4_24

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  • DOI: https://doi.org/10.1007/978-1-0716-1884-4_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1883-7

  • Online ISBN: 978-1-0716-1884-4

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