Abstract
The recent development in genetic research indicates that there exists intraspecific genetic variability in many organism groups. These variations, which result in a variety of genotypes and phenotypes within a population, are called polymorphism. Mutations in different ways can alter the organism’s phenotype and affect its fitness, for example, by altering disease susceptibility or resistance. Therefore, the detection of point mutations in different genes of a population is of particular importance. The amplification-refractory mutation system technique is a PCR-based method to detect single nucleotide polymorphisms in the genome. The high repeatability, low cost, high accessibility, and no need for sophisticated technology are the main advantages of the ARMS-PCR technique, compared with other available methods such as PCR-RFLP. This chapter describes the design and analysis method of primers for the ARMS-PCR technique.
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References
Shastry BS (2002) SNP alleles in human disease and evolution. J Hum Genet 47(11):0561–0566
Bai R-K, Wong L-JC (2004) Detection and quantification of heteroplasmic mutant mitochondrial DNA by real-time amplification refractory mutation system quantitative PCR analysis: a single-step approach. Clin Chem 50(6):996–1001
Matsuda K (2017) PCR-based detection methods for single-nucleotide polymorphism or mutation: real-time PCR and its substantial contribution toward technological refinement. In: Advances in clinical chemistry, vol 80. Elsevier, Amsterdam, pp 45–72
Yang L, Ijaz I, Cheng J, Wei C, Tan X, Khan MA et al (2018) Evaluation of amplification refractory mutation system (ARMS) technique for quick and accurate prenatal gene diagnosis of CHM variant in choroideremia. Appl Clin Genet 11:1
Makanga JO, Christianto A, Inazu T (2015) Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection. In: PCR primer design. Springer, New York, pp 117–125
Medrano RFV, de Oliveira CA (2014) Guidelines for the tetra-primer ARMS–PCR technique development. Mol Biotechnol 56(7):599–608
Ye S, Dhillon S, Ke X, Collins AR, Day IN (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res 29(17):e88
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Komijani, M., Shahin, K., Azhar, E.I., Bahram, M. (2022). Designing PCR Primers for the Amplification-Refractory Mutation System. In: Basu, C. (eds) PCR Primer Design. Methods in Molecular Biology, vol 2392. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1799-1_7
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DOI: https://doi.org/10.1007/978-1-0716-1799-1_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1798-4
Online ISBN: 978-1-0716-1799-1
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