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Development of CPP-Based Methods for Delivery of miRNAs into the Skin and Airways: Lessons from Cell Culture and Mouse Models

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Cell Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2383))

Abstract

microRNAs (miRNAs) have capacity to modulate numerous biological processes and therefore synthetic oligonucleotides mimicking or inhibiting particular miRNA have potential in the development of novel types of therapeutics. We have elaborated several methods for safe and efficient overexpression of miRNAs using self-forming nanocomplexes of PepFect or NickFect type of cell-penetrating peptides (CPPs) and oligonucleotides mimicking well-characterized anti-inflammatory miR-146a. We focus on chronic inflammatory diseases affecting epithelium, such as atopic dermatitis and asthma harnessing respective cell cultures and mouse models. Here we provide protocols for miRNA transfection into primary human keratinocytes, primary human bronchial epithelial cells, and human monocyte-derived dendritic cells using CPPs PepFect14, NickFect70, NickFect71, and NickFect72. In addition, we provide protocols for application of CPP-miRNA nanocomplexes in in vivo mouse models of irritant contact dermatitis and allergic airway inflammation.

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Acknowledgments

This work was supported by European Regional Development Fund (Project No. 2014-2020.4.01.15-0012) from European Union, personal (PUT1669 for AR, PUT1617 for MP) from Estonian Research Council, Leo Foundation grant (LF17040) and Institute of Technology basic financing grant (PLTTI20912 to MP).

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Authors and Affiliations

  1. Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia

    Anet Laanesoo, Kapilraj Periyasamy & Ana Rebane

  2. Institute of Technology, University of Tartu, Tartu, Estonia

    Margus Pooga

Authors
  1. Anet Laanesoo
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  2. Kapilraj Periyasamy
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  3. Margus Pooga
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  4. Ana Rebane
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Corresponding author

Correspondence to Ana Rebane .

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Editors and Affiliations

  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Ãœlo Langel

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Laanesoo, A., Periyasamy, K., Pooga, M., Rebane, A. (2022). Development of CPP-Based Methods for Delivery of miRNAs into the Skin and Airways: Lessons from Cell Culture and Mouse Models. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_32

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  • DOI: https://doi.org/10.1007/978-1-0716-1752-6_32

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1751-9

  • Online ISBN: 978-1-0716-1752-6

  • eBook Packages: Springer Protocols

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