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Analyze Mouse Knockout Models of UPR Pathway Elements

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The Unfolded Protein Response

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2378))

Abstract

Evidence from genetic studies in human and mice indicates that defective skeletal development is one of the major phenotypic outcomes for aberrant UPR signaling. Visualization of morphological alterations in whole-mount skeleton and protein secretion and UPR activation on tissue sections is the very first step to investigate skeletal phenotypes of UPR-related mouse models. In this chapter, we introduce the major techniques that have been frequently used in our laboratory to study UPR-induced skeletal disorders with genetically modified mice and provide descriptive directions of mouse genotyping, bone tissue grossing, whole-mount skeletal staining, immunostaining assays of matrix secretion, and UPR activation.

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Acknowledgments

We would like to thank Dr. Kathryn S.E.Cheah for the ColX antibody . This work was supported by the National Natural Science Foundation of China (81871743, 82002261, and 81972032) and the Shaanxi Innovation Team Project (2020TD-036).

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Authors and Affiliations

  1. Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, China

    Chao Zheng & Liu Yang

  2. School of Biomedical Sciences, University of Hong Kong, Pok Fu Lam, Hong Kong, China

    Cheng Wang

  3. Department of Orthopedic Surgery, HongHui Hospital, Xi’an Jiaotong University, College of Medicine, Xi’an, China

    Qiang Jie

Authors
  1. Chao Zheng
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  2. Cheng Wang
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  3. Qiang Jie
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  4. Liu Yang
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Corresponding authors

Correspondence to Qiang Jie or Liu Yang .

Editor information

Editors and Affiliations

  1. Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Paterna, Spain

    Roberto Pérez-Torrado

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zheng, C., Wang, C., Jie, Q., Yang, L. (2022). Analyze Mouse Knockout Models of UPR Pathway Elements. In: Pérez-Torrado, R. (eds) The Unfolded Protein Response. Methods in Molecular Biology, vol 2378. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1732-8_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1732-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1731-1

  • Online ISBN: 978-1-0716-1732-8

  • eBook Packages: Springer Protocols

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