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Screening for Anticancer Activity: DNA Fragmentation Assay

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Methods in Actinobacteriology

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Cancer is the world’s second major cause of death, and despite significant advances in drug distribution, bacteria-based therapies still need to be developed. Bacterial cell free supernatant (CFS) remedies have many advantages relative to the use of traditional therapeutic agents to mitigate the side effects caused by therapies. The objective of the protocol was to discover the possible cytotoxicity of CFS, ethanol extract and its derived fractions (chloroform, ethyl acetate, butanol, and aqueous). DNA fragmentation assay methodology was applied to determine the anticancer activity in human cell lines (NCI-H460, HepG2).

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Author information

Authors and Affiliations

  1. Departmentof Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan

    InamulHasan Madar

  2. Departmentof Computer Science, Faculty of Science, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Ghazala Sultan

  3. Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do, South Korea

    Ramachandran Chelliah & Deog-Hwan Oh

Authors
  1. InamulHasan Madar
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  2. Ghazala Sultan
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  3. Ramachandran Chelliah
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  4. Deog-Hwan Oh
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Editor information

Editors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Madar, I., Sultan, G., Chelliah, R., Oh, DH. (2022). Screening for Anticancer Activity: DNA Fragmentation Assay. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_58

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_58

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

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