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Measuring Chromatin Accessibility: ATAC-Seq

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Enhancers and Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2351))

Abstract

Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) is a method to investigate the accessibility of chromatin in a genome-wide fashion. In this chapter, we provide a brief history of the chromatin accessibility field followed by a detailed protocol to perform ATAC-Seq assay.

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Acknowledgments

We would like to thank the members of the Tiwari lab for their cooperation and critical feedback. Importantly, we are very much thankful to Dr. Benedetto Daniele Giaimo for his critical suggestions in shaping this chapter.

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Author notes

  1. Sanjeeb Kumar Sahu and Amitava Basu contributed equally.

Authors and Affiliations

  1. Salk Institute for Biological Studies, La Jolla, CA, USA

    Sanjeeb Kumar Sahu

  2. Institute of Molecular Biology (IMB), Mainz, Germany

    Amitava Basu

  3. Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry & Biomedical Science, Queen’s University Belfast, Belfast, UK

    Vijay K. Tiwari

Authors
  1. Sanjeeb Kumar Sahu
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  2. Amitava Basu
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  3. Vijay K. Tiwari
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Corresponding author

Correspondence to Vijay K. Tiwari .

Editor information

Editors and Affiliations

  1. Institute of Biochemistry, University of Giessen, Gießen, Hessen, Germany

    Tilman Borggrefe

  2. Institute of Biochemistry, University of Giessen, Gießen, Hessen, Germany

    Benedetto Daniele Giaimo

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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sahu, S.K., Basu, A., Tiwari, V.K. (2021). Measuring Chromatin Accessibility: ATAC-Seq . In: Borggrefe, T., Giaimo, B.D. (eds) Enhancers and Promoters. Methods in Molecular Biology, vol 2351. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1597-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1597-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1596-6

  • Online ISBN: 978-1-0716-1597-3

  • eBook Packages: Springer Protocols

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