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Experimental Validation of the Noncoding Potential for lncRNAs

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Long Non-Coding RNAs in Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2348))

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Abstract

In recent years, long noncoding RNAs (lncRNAs) have been increasingly recognized as critical regulators of a broad spectrum of cellular processes. Recent advancements in proteomic technologies have uncovered that an abundance of noncoding genes, including lncRNAs, have been misannotated and in reality encode proteins. This revelation underscores the need to accurately determine the coding potential of lncRNAs prior to assessment of their functional mechanisms. Here, we detail numerous experimental techniques useful in the determination of lncRNA coding potential. Several of these methods are doubly useful in that they may also be employed in studying the function of a lncRNA, be it via an RNA, protein, or both.

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Acknowledgments

This research to A.L and E.D was supported by the Intramural Research Program of the National Cancer Institute (NCI), Center for Cancer Research (CCR), National Institutes of Health (NIH).

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Authors and Affiliations

  1. Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA

    Emily A. Dangelmaier & Ashish Lal

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  1. Emily A. Dangelmaier
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  2. Ashish Lal
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Corresponding author

Correspondence to Ashish Lal .

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Editors and Affiliations

  1. Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain

    Alfons Navarro

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Dangelmaier, E.A., Lal, A. (2021). Experimental Validation of the Noncoding Potential for lncRNAs. In: Navarro, A. (eds) Long Non-Coding RNAs in Cancer. Methods in Molecular Biology, vol 2348. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1581-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1581-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1580-5

  • Online ISBN: 978-1-0716-1581-2

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