Abstract
G protein-coupled receptors (GPCRs) constitute the largest family of plasma membrane receptors, thus representing the more investigated drug targets in the design of new pharmacotherapeutic strategies. In this family of receptors, the binding of an agonist typically triggers the activation of heterotrimeric G proteins, which in turn control the propagation of secondary messenger molecules, such as cyclic adenosine monophosphate (cAMP), which play a key role in important physiological processes. Accordingly, determining GPCR-mediated cAMP accumulation in native tissue (i.e., synaptosomes) constitutes an important step in the pharmacological characterization of these receptors. Here, we describe the methodology used to assess GPCR-mediated cAMP accumulation in rat striatal synaptosomes.
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Acknowledgments
This work was supported by Ministerio de Ciencia, Innovación y Universidades–Agencia Estatal de Investigación-FEDER-UE (SAF2017-87349-R MICIU/AEI/FEDER/UE) and Generalitat de Catalunya (2017SGR1604). We thank Centres de Recerca de Catalunya (CERCA) Programme/Generalitat de Catalunya for IDIBELL institutional support. We thank E. Castaño and B. Torrejón from the Scientific and Technical Services (SCT) group at the Bellvitge Campus of the University of Barcelona for their technical assistance.
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Taura, J., Fernández-Dueñas, V., Ciruela, F. (2021). Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes. In: Lujan, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1522-5_32
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DOI: https://doi.org/10.1007/978-1-0716-1522-5_32
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