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Methods for Proteomic Analyses of Mycobacteria

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2314))

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Abstract

The use of proteomic technologies to characterize and study the proteome of mycobacteria has provided important information in terms of function, diversity, protein–protein interactions, and host-pathogen interactions in Mycobacterium spp. There are many different mass spectrometry methodologies that can be applied to proteomics studies of mycobacteria and microorganisms in general. Sample processing and appropriate study design are critical to generating high-quality data regardless of the mass spectrometry method applied. Appropriate study design relies on statistical rigor and data curation using bioinformatics approaches that are widely applicable regardless of the organism or system studied. Sample processing, on the other hand, is often a niched process specific to the physiology of the organism or system under investigation. Therefore, in this chapter, we will provide protocols for processing mycobacterial protein samples for the specific application of Top-down and Bottom-up proteomic analyses.

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Acknowledgement

This work was supported by ATCC contract 2010-0516-0005 (a subcontract of NIH-NIAID Contract HHSN272201000027c) and ATCC contract 2016-0550-0002 (a subcontract of NIH-NIAID contract HHSN272201600013c.

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Authors and Affiliations

  1. Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA

    Carolina Mehaffy, Megan Lucas, Nicole A. Kruh-Garcia & Karen M. Dobos

  2. BioMARC, Colorado State University, Fort Collins, CO, USA

    Nicole A. Kruh-Garcia

Authors
  1. Carolina Mehaffy
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  2. Megan Lucas
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  3. Nicole A. Kruh-Garcia
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  4. Karen M. Dobos
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Corresponding author

Correspondence to Karen M. Dobos .

Editor information

Editors and Affiliations

  1. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Tanya Parish

  2. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Anuradha Kumar

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Mehaffy, C., Lucas, M., Kruh-Garcia, N.A., Dobos, K.M. (2021). Methods for Proteomic Analyses of Mycobacteria. In: Parish, T., Kumar, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 2314. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1460-0_23

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  • DOI: https://doi.org/10.1007/978-1-0716-1460-0_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1459-4

  • Online ISBN: 978-1-0716-1460-0

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