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Neuronal Cell Culture

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Shohreh Amini

    1. College of Science and Technology, Temple University, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Martyn K. White

    1. Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2311)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. General Overview of Neuronal Cell Culture

    • Jennifer Gordon, Shohreh Amini
    Pages 1-8

  3. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology

    • Jane Kovalevich, Maryline Santerre, Dianne Langford
    Pages 9-23

  4. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12, and SK-N-MC

    • Nune Darbinian
    Pages 25-38

  5. Murine Teratocarcinoma-Derived Neuronal Cultures

    • Datta Prasun K.
    Pages 39-49

  6. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons

    • Armine Darbinyan, Rafal Kaminski, Martyn K. White, Paul D. Pozniak, Nune Darbinian, Kamel Khalili
    Pages 51-61

  7. Mouse Enteric Neuronal Cell Culture

    • Yonggang Zhang, Wenhui Hu
    Pages 63-71

  8. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications

    • Lyandysha V. Zholudeva, Ying Jin, Liang Qiang, Michael A. Lane, Itzhak Fischer
    Pages 73-108

  9. Slice Culture Modeling of CNS Viral Infection

    • Kalen R. Dionne, Kenneth L. Tyler, Penny Clarke
    Pages 109-130

  10. Neurospheres and Glial Cell Cultures; from Plating to Cell Phenotyping

    • Amanda Parker Struckhoff, Luis Del Valle
    Pages 131-145

  11. Transfection of Neuronal Cultures

    • Ilker Kudret Sariyer
    Pages 147-153

  12. Lentiviral Transduction of Neuronal Cells

    • Anna Bellizzi, Nicholas Ahye, Hassen S. Wollebo
    Pages 155-160

  13. Compartmentalized Neuronal Cultures

    • Armine Darbinyan, Paul D. Pozniak, Nune Darbinian, Martyn K. White, Kamel Khalili
    Pages 161-166

  14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates

    • George M. Smith, Yingpeng Liu
    Pages 167-175

  15. Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents

    • Masoud Shekarabi, Jake A. Robinson, Tricia H. Burdo
    Pages 177-184

  16. Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection

    • Nune Darbinian, Armine Darbinyan, Nana Merabova, Shohreh Amini
    Pages 185-193

  17. Fetal Brain Injury Models of Fetal Alcohol Syndrome : Examination of Neuronal Morphologic Condition Using Sholl Assay

    • Nune Darbinian, Armine Darbinyan, Kamel Khalili, Shohreh Amini
    Pages 195-201

  18. Back Matter

    Pages 203-205
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Keywords

About this book

This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved.  Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.  

Editors and Affiliations

  • College of Science and Technology, Temple University, Philadelphia, USA

    Shohreh Amini

  • Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, USA

    Martyn K. White

Bibliographic Information

  • Book Title: Neuronal Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Shohreh Amini, Martyn K. White

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1437-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1436-5Published: 26 May 2021

  • Softcover ISBN: 978-1-0716-1439-6Published: 26 May 2021

  • eBook ISBN: 978-1-0716-1437-2Published: 25 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 205

  • Number of Illustrations: 8 b/w illustrations, 42 illustrations in colour

  • Topics: Neurosciences

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