Structure and Function of Membrane Proteins

1. Front Matter

   Pages i-xii

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2. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL

   • Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, M. Joanne Lemieux

   Pages 1-20

3. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies

   • Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, Ingeborg Schmidt-Krey

   Pages 21-35

4. Biochemical Characterization of GPCR–G Protein Complex Formation

   • Filip Pamula, Ching-Ju Tsai

   Pages 37-48

5. Electrophysiological Approaches for the Study of Ion Channel Function

   • Guiying Cui, Kirsten A. Cottrill, Nael A. McCarty

   Pages 49-67

6. Isothermal Titration Calorimetry of Membrane Proteins

   • Han N. Vu, Alan J. Situ, Tobias S. Ulmer

   Pages 69-79

7. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level

   • Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, Gavin M. King

   Pages 81-99

8. Structure Determination of Membrane Proteins Using X-Ray Crystallography

   • Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, Nicholas Noinaj

   Pages 101-136

9. Studying Membrane Protein Structures by MicroED

   • Michael W. Martynowycz, Tamir Gonen

   Pages 137-151

10. Single-Particle Cryo-EM of Membrane Proteins

    • Dovile Januliene, Arne Moeller

    Pages 153-178

11. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy

    • Mary D. Hernando, Joseph O. Primeau, Howard S. Young

    Pages 179-199

12. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel

    • Robert Silvers, Matthew T. Eddy

    Pages 201-217

13. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering

    • Yuqi Wu, Kevin L. Weiss, Raquel L. Lieberman

    Pages 219-235

14. Preparing Membrane Proteins for Simulation Using CHARMM-GUI

    • Yupeng Li, Jinchan Liu, James C. Gumbart

    Pages 237-251

15. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide

    • William G. Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways et al.

    Pages 253-273

16. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins

    • Yandong Huang, Jack A. Henderson, Jana Shen

    Pages 275-287

17. Molecular Dynamics–Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions

    • Dylan Ogden, Mahmoud Moradi

    Pages 289-309

18. Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations

    • Wesley M. Botello-Smith, Yun Lyna Luo

    Pages 311-334

19. Large-Scale Molecular Dynamics Simulations of Cellular Compartments

    • Eric Wilson, John Vant, Jacob Layton, Ryan Boyd, Hyungro Lee, Matteo Turilli et al.

    Pages 335-356

20. Back Matter

    Pages 357-358

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This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. 

Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to furtherunderstand these essential proteins and their many biological roles.

• Over-expression
• Phospholipid bilayers
• Protein purification
• Functional studies
• Protein structure

• School of Biological Sciences and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA

  Ingeborg Schmidt-Krey

• School of Physics, Georgia Institute of Technology, Atlanta, USA

  James C. Gumbart

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