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Co-immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants

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Plant Photomorphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2297))

Abstract

Co-immunoprecipitation (CoIP) assay has been used as a powerful and routine technique to detect in vivo protein-protein interactions. Not only can it probe stable interactions, but also it is applicable for semiquantitative and inducible protein associations. Here we describe the protocol for detecting blue light-dependent protein interactions, particularly for blue light receptor cryptochrome-mediated complex formation. In addition, we present some notes which may be helpful for common Co-IP study as well.

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Acknowledgments

We thank Dr. Danmeng Zhu and Dr. Yuqiu Wang from Peking University, China for valuable advising on CoIP assay.

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, John Innes Centre, Norwich, UK

    Jingyi Zhang & Shengbo He

Authors
  1. Jingyi Zhang
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  2. Shengbo He
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Corresponding author

Correspondence to Shengbo He .

Editor information

Editors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    Ruohe Yin

  2. Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    Ling Li

  3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    Kaijing Zuo

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Zhang, J., He, S. (2021). Co-immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants. In: Yin, R., Li, L., Zuo, K. (eds) Plant Photomorphogenesis. Methods in Molecular Biology, vol 2297. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1370-2_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1370-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1369-6

  • Online ISBN: 978-1-0716-1370-2

  • eBook Packages: Springer Protocols

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