Abstract
The intergeneric hybridization of wheat (Triticum aestivum L.) with maize (Zea mays L.) enables the production of doubled haploids (DHs) of wheat from all wheat hybrids with high efficiencies. Wheat and maize donor plants are raised in environmentally controlled greenhouses until crossing. Before anthesis, wheat spikes are emasculated and then pollinated with maize. Auxin is applied to each individual wheat floret 1 day after pollination. About 2 weeks after crossing, in vitro embryo culture is performed, enabling the regeneration of haploid wheat plantlets after maize chromosome elimination. Haploid plantlets are transferred to the greenhouse and after recovery, their genome is doubled with colchicine. Haploid plantlets can be sampled for DNA extractions and molecular analyses to aid the rapid discard of undesirable plantlets. Doubled haploid plants are raised in a greenhouse until maturity. Seeds of each fertile DH are harvested and often sown the same year. Several cycles of multiplication and evaluation in replicated plot trials and different geographical locations are then done to select the best candidate(s) for varietal registration.
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Acknowledgments
The author gratefully acknowledges contributions of colleagues and technical support to the development over the years of the described protocol. Many thanks are due to Dr. R.A. Pickering for critically reviewing this manuscript. This article is dedicated to the memory of Mr. Michel Desprez, breeder and scientist, former Research Director at Florimond Desprez.
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Devaux, P. (2021). Production of Wheat Doubled Haploids Through Intergeneric Hybridization with Maize. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_14
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DOI: https://doi.org/10.1007/978-1-0716-1315-3_14
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