Abstract
Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4+ T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.
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Siracusa, F., Lahmann, A., Vanni, A., Cortesi, F., Mazzoni, A. (2021). Human and Murine T-Helper Cell Recovery from Organs and Tissues. In: Annunziato, F., Maggi, L., Mazzoni, A. (eds) T-Helper Cells. Methods in Molecular Biology, vol 2285. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1311-5_1
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DOI: https://doi.org/10.1007/978-1-0716-1311-5_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1310-8
Online ISBN: 978-1-0716-1311-5
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