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Assessment of the Effects of Drugs on Mitochondrial Respiration

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2277))

Abstract

Mitochondria are targets of newly synthesized drugs and being tested for the treatment of various diseases caused or accompanied by disruption of cellular bioenergetics. In drug development, it is necessary to test for drug-induced changes in mitochondrial enzyme activity that may be related to therapeutic or adverse drug effects. Measurement of drug effect on mitochondrial oxygen consumption kinetics and/or protective effects of drugs against calcium-induced inhibition of the mitochondrial respiration can be used for the study mitochondrial toxicity and neuroprotective effects of drugs. Supposing that the drug-induced inhibition of the mitochondrial respiratory rate and/or individual mitochondrial complexes is associated with adverse drug effects, the effects of drugs on mitochondrial respiration in isolated mitochondria allow selection of novel molecules that are relatively safe for mitochondrial toxicity.

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Acknowledgments

This work was supported by the Charles University [project Progres Q27/LF1] and the Czech Science Foundation [grant numbers 17-05292S and 17-07585Y], Czech Republic. The authors thank Zdeněk Hanuš for providing careful technical assistance.

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Authors and Affiliations

  1. First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic

    Jana Hroudová & Zdeněk Fišar

Authors
  1. Jana Hroudová
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  2. Zdeněk Fišar
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Corresponding author

Correspondence to Zdeněk Fišar .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, Midwestern University, Glendale, AZ, USA

    Volkmar Weissig

  2. Cochin Hospital, Cochin Institute, INSERM U1016, PARIS, France

    Marvin Edeas

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Hroudová, J., Fišar, Z. (2021). Assessment of the Effects of Drugs on Mitochondrial Respiration. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 2277. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1270-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1270-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1269-9

  • Online ISBN: 978-1-0716-1270-5

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