Abstract
The in vitro production of platelets could provide a life-saving intervention for patients that would otherwise require donor-derived platelets. Producing large numbers of platelets in vitro from their progenitor cells, megakaryocytes, remains remarkably difficult and inefficient. Here, a human megakaryoblast leukemia cell line (MEG-01) was used to assess the maturation of megakaryocytes and to develop a new methodology for producing high numbers of platelet-like particles from mature MEG-01 cells in vitro.
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Acknowledgments
The authors gratefully acknowledge the funding sources to support this work from the National Science Foundation CAREER Program (CBET-1554017), the Office of Naval Research Young Investigator Program (N00014-16-1-3012), the National Institutes of Health Trailblazer Award (1R21EB025413-01), the National Institutes of Health Director’s New Innovator Award (1DP2CA250006-01), and the Undergraduate Research Opportunity Program (awarded to KP and ML). This work was also supported by the University of Utah Flow Cytometry Facility, the University of Utah Fluorescence Microscopy Core Facility (1S10RR024761-01), the National Cancer Institute (5P30CA042014-24), and the National Center for Research of the National Institutes of Health (1S10RR026802-01).
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Persson, K.M., Kneller, P.V., Livingston, M.W., Bush, L.M., Deans, T.L. (2021). High-Throughput Production of Platelet-Like Particles. In: Ebrahimkhani, M.R., Hislop, J. (eds) Programmed Morphogenesis. Methods in Molecular Biology, vol 2258. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1174-6_18
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DOI: https://doi.org/10.1007/978-1-0716-1174-6_18
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