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Monitoring Bacterial Translation Rates Genome-Wide

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Ribosome Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2252))

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Abstract

Modern DNA sequencing technologies have allowed for the sequencing of tens of thousands of bacterial genomes. While this explosion of information has brought about new insights into the diversity of the prokaryotic world, much less is known of the identity of proteins encoded within these genomes, as well as their rates of production. The advent of ribosome profiling, or the deep sequencing of ribosome-protected footprints, has recently enabled the systematic evaluation of every protein-coding region in a given experimental condition, the rates of protein production for each gene, and the variability in translation rates across each message. Here, I provide an update to the bacterial ribosome profiling approach, with a particular emphasis on a simplified strategy to reduce cloning time.

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Acknowledgments

I thank Emily Powers and Gloria Brar for use of reagents and equipment.

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Authors and Affiliations

  1. Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA

    Eugene Oh

  2. Department of Medicine, Harvard Medical School, Boston, MA, USA

    Eugene Oh

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  1. Eugene Oh
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Correspondence to Eugene Oh .

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Editors and Affiliations

  1. Department of Dermatology, Boston University School of Medicine, Boston, MA, USA

    Vyacheslav M. Labunskyy

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Oh, E. (2021). Monitoring Bacterial Translation Rates Genome-Wide. In: Labunskyy, V.M. (eds) Ribosome Profiling. Methods in Molecular Biology, vol 2252. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1150-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1150-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1149-4

  • Online ISBN: 978-1-0716-1150-0

  • eBook Packages: Springer Protocols

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