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Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat

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The Plant Microbiome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2232))

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Abstract

Beneficial plant-microbe interactions are important and desirable for sustainable intensification of agriculture. Here, we describe methods to isolate microbes from the roots of field-grown wheat plants. This includes the rhizosphere and rhizoplane soil, as well as the root endosphere. We also describe a method to test for endosphere competence of putative endophytes.

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References

  1. Poole P, Ramachandran V, Terpolilli J (2018) Rhizobia: from saprophytes to endosymbionts. Nat Rev Microbiol 16(5):291–303. https://doi.org/10.1038/nrmicro.2017.171

    Article  CAS  PubMed  Google Scholar 

  2. Bonfante P, Genre A (2010) Mechanisms underlying beneficial plant-fungus interactions in mycorrhizal symbiosis. Nat Commun 1:48. https://doi.org/10.1038/ncomms1046

    Article  CAS  PubMed  Google Scholar 

  3. Compant S, Samad A, Faist S et al (2019) A review on the plant microbiome: ecology, functions, and emerging trends in microbial application. J Adv Res 19:29–37. https://doi.org/10.1016/j.jare.2019.03.004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Hirsch PR, Mauchline TH (2012) Who’s who in the plant root microbiome? Nat Biotechnol 30(10):961–962. https://doi.org/10.1038/nbt.2387

    Article  CAS  PubMed  Google Scholar 

  5. Mauchline TH, Malone JG (2017) Life in earth—the root microbiome to the rescue? Curr Opin Microbiol 37:23–28. https://doi.org/10.1016/j.mib.2017.03.005

    Article  PubMed  Google Scholar 

  6. Robinson RJ, Fraaije BA, Clark IM et al (2016) Endophytic bacterial composition in wheat (Triticum aestivum) is determined by plant tissue type, developmental stage and soil nutrient availability. Plant Soil 4:1–6. https://doi.org/10.1007/s11104-015-2495-4

    Article  CAS  Google Scholar 

  7. Robinson RJ, Fraaije BA, Clark IM et al (2016) Wheat seed embryo excision enables the creation of axenic seedlings and Koch’s postulates testing of putative bacterial endophytes. Sci Rep 6:25581. https://doi.org/10.1038/srep25581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Mauchline TH, Chedom-Fotso D, Chandra G et al (2015) An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota. Environ Microbiol 17(11):4764–4778. https://doi.org/10.1111/1462-2920.13038

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, Herts, UK

    Rebekah J. Robinson, Vanessa N. Kavamura, Penny R. Hirsch, Ian M. Clark & Tim H. Mauchline

Authors
  1. Rebekah J. Robinson
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  2. Vanessa N. Kavamura
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  3. Penny R. Hirsch
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  4. Ian M. Clark
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  5. Tim H. Mauchline
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Corresponding author

Correspondence to Tim H. Mauchline .

Editor information

Editors and Affiliations

  1. Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia

    Lilia C. Carvalhais

  2. School of Earth and Environmental Sciences, The University of Queensland, Brisbane, QLD, Australia

    Paul G. Dennis

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Robinson, R.J., Kavamura, V.N., Hirsch, P.R., Clark, I.M., Mauchline, T.H. (2021). Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat. In: Carvalhais, L.C., Dennis, P.G. (eds) The Plant Microbiome. Methods in Molecular Biology, vol 2232. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1040-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1040-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1039-8

  • Online ISBN: 978-1-0716-1040-4

  • eBook Packages: Springer Protocols

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