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Sequencing of Complete Chloroplast Genomes

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2222))

Abstract

In this chapter, frequently used methods for elucidating sequence and structure of chloroplast genomes are reviewed, as a current best practice guide. This concerns methods for DNA extraction, sequencing library preparation, and bioinformatics (assembly, verification, annotation, and sequence comparisons). Recommendations for standard data reporting practices are given—chloroplast genome sequencing reports can be highly formalized, and publication in the form of standard data reports is the best option for comparison and meta-analysis purposes.

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Authors and Affiliations

  1. Department of Genetics, Austrian Federal Research Centre for Forests (BFW), Vienna, Austria

    Berthold Heinze

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  1. Berthold Heinze
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Correspondence to Berthold Heinze .

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  1. UMR PVBMT, Universite de la Reunion, St Pierre, RĂ©union, France

    Pascale Besse

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Database (Excel table) of articles describing methods in chloroplast genome sequencing (XLSX 42 kb)

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Heinze, B. (2021). Sequencing of Complete Chloroplast Genomes. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 2222. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0997-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0997-2_5

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