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Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

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Listeria Monocytogenes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2220))

Abstract

The behavior of Listeria monocytogenes communities in the food chain is closely associated with their spatial organization. Whether as biofilms on industrial surfaces or as microcolonies in food matrices, the resulting physiological diversification combined with the presence of extracellular polymeric substances (EPS) triggers emergent community functions involved in the pathogen survival and persistence (e.g., tolerance to dehydration, biocides, or preservatives). In this contribution, we present a noninvasive confocal laser microscopy (CLM) protocol allowing exploration of the spatial organization of L. monocytogenes communities on various inert or nutritive materials relevant for the food industry.

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Acknowledgments

This project was funded by the French National Agency for Research (program “PathoFood ANR-17-CE21-0002”) and by AgroParisTech (program “SBAO19-2”). Microscopic images were performed at the INRA MIMA2 imaging center. We thank Kevin Bascaran for technical assistance.

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Authors and Affiliations

  1. Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

    Maud Darsonval, Marina Grégoire, Julien Deschamps & Romain Briandet

Authors
  1. Maud Darsonval
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  2. Marina Grégoire
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  3. Julien Deschamps
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  4. Romain Briandet
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Corresponding author

Correspondence to Romain Briandet .

Editor information

Editors and Affiliations

  1. Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK

    Edward M. Fox

  2. INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France

    Hélène Bierne

  3. Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria

    Beatrix Stessl

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Darsonval, M., Grégoire, M., Deschamps, J., Briandet, R. (2021). Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities. In: Fox, E.M., Bierne, H., Stessl, B. (eds) Listeria Monocytogenes. Methods in Molecular Biology, vol 2220. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0982-8_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0982-8_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0981-1

  • Online ISBN: 978-1-0716-0982-8

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