Abstract
Multielectrode arrays (MEAs) are grids of substrate-integrated microelectrodes that allow for electrophysiological interrogation of dissociated cell cultures or tissue slices. Here we discuss the use of nonimplantable electrodes for studies. The methods described attempt to provide a starting point for researchers new to the field who wish to begin to utilize this powerful, but daunting technology and quickly apply the basic principles to their own research interests.
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References
Pine J (2006) A history of MEA development. In: Taketani M, Baudry M (eds) Advances in network electrophysiology using multi-electrode arrays. Springer, New York
Lein PJ, Barnhart CD, Pessah IN (2012) Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol Biol 758:115–134
Humpel C (2015) Organotypic brain slice cultures: a review. Neuroscience 305:86–98
Berdichevsky Y (2009) Microfluidics and multielectrode array-compatible organotypic slice culture method. J Neurosci Methods 178(1):59–64
Plenz D (2011) Multi-electrode array recordings of neuronal avalanches in organotypic cultures. J Vis Exp 54:e2949. https://doi.org/10.3791/2949
Johnstone AFM (2010) Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology 31:331–350
Stett A (2003) Biological application of microelectrode arrays in drug discovery and basic research. Anal Bioanal Chem 377:486–495
Spijker S (2011) Dissection of rodent brain regions. In: Li KW (ed) Neuroproteomics. Humana Press, New York/Heidelberg, pp Â13–26. https://doi.org/10.1007/978-1-61779-111-6_2
Dreyfus CF, Black IB (1990) Multiple approaches to brain culture. In: Conn PM (ed) Cell culture methods in neuroscience,vol 2. Elsevier, pp 3–16. https://doi.org/10.1016/B978-0-12-185254-2.50005-X
Pacifici M, Peruzzi F (2012) Isolation and culture of rat embryonic neural cells: a quick protocol. J Vis Exp 63:e3965. https://doi.org/10.3791/3965
Huettner JE, Baughmann RW (1986) Primary culture of identified neurons from the visual cortex of postnatal rats. J Neurosci 6(10):3044–3060
Finkbeiner S, Stevens CF (1988) Application of quantitative measurements for assessing glutamate neurotoxicity. Proc Natl Acad Sci U S A 85:4071–4074
Papouin T, Haydon PG (2018) Obtaining acute brain slices. Bio Protoc 8(2):e2699. https://doi.org/10.21769/BioProtoc.2699
Ting JT, Daigle TL, Chen Q et al (2014) Acute brain slice methods for adult and aging animals: application of targeted patch clamp analysis and optogenetics. Methods Mol Biol 1183:221–242
Kessler M (2008) Spontaneous activity in Purkinje cells: multi-electrode recording from organotypic cerebellar slice cultures. Brain Res 1218:54–69
Xiong G, Metheny H, Johnson BN et al (2017) A comparison of different slicing planes in preservation of major hippocampal pathway fibers in the mouse. Front Neuroanat 11:107. https://doi.org/10.3389/fnana.2017.00107. eCollection 2017
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Burley, R., Harvey, J.R.M. (2021). Multielectrode Arrays. In: Dallas, M., Bell, D. (eds) Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0818-0_6
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DOI: https://doi.org/10.1007/978-1-0716-0818-0_6
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