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The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer

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Immunometabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2184))

Abstract

Metabolism plays an important role in the activation and effector functions of macrophages. Intracellular pathogens, such as Mycobacterium tuberculosis, subvert the immune functions of macrophages to establish an infection by modulating the metabolism of the macrophage. Here, we describe how the Seahorse Extracellular Flux Analyzer (XF) from Agilent Technologies can be used to study the changes in the bioenergetic metabolism of the macrophages induced by infection with mycobacteria. The XF simultaneously measures the oxygen consumption and extracellular acidification of the macrophages noninvasively in real time, and together with the addition of metabolic modulators, substrates, and inhibitors enables measurements of the rates of oxidative phosphorylation, glycolysis, and ATP production.

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Author information

Authors and Affiliations

  1. Africa Health Research Institute, Durban, South Africa

    Bridgette M. Cumming & Adrie J. C. Steyn

  2. Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA

    Vineel P. Reddy & Adrie J. C. Steyn

  3. UAB Centers for AIDS Research and Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    Adrie J. C. Steyn

Authors
  1. Bridgette M. Cumming
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  2. Vineel P. Reddy
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  3. Adrie J. C. Steyn
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Corresponding author

Correspondence to Adrie J. C. Steyn .

Editor information

Editors and Affiliations

  1. Faculty of Health Sciences, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada

    Suresh Mishra

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Cumming, B.M., Reddy, V.P., Steyn, A.J.C. (2020). The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer. In: Mishra, S. (eds) Immunometabolism. Methods in Molecular Biology, vol 2184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0802-9_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0802-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0801-2

  • Online ISBN: 978-1-0716-0802-9

  • eBook Packages: Springer Protocols

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