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Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers

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Vaccine Delivery Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2183))

Abstract

Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D).

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Acknowledgments

This work was supported by the Nanotechnology Research Center and the Human Health Therapeutics Research Center at the National Research Council of Canada.

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Authors and Affiliations

  1. Nanotechnology Research Center, National Research Council Canada, Edmonton, AB, Canada

    Hui Qian

  2. Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada

    Yimei Jia & Michael J. McCluskie

Authors
  1. Hui Qian
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  2. Yimei Jia
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  3. Michael J. McCluskie
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Corresponding author

Correspondence to Michael J. McCluskie .

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Editors and Affiliations

  1. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Blaine A. Pfeifer

  2. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Andrew Hill

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Qian, H., Jia, Y., McCluskie, M.J. (2021). Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers. In: Pfeifer, B.A., Hill, A. (eds) Vaccine Delivery Technology. Methods in Molecular Biology, vol 2183. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0795-4_28

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  • DOI: https://doi.org/10.1007/978-1-0716-0795-4_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0794-7

  • Online ISBN: 978-1-0716-0795-4

  • eBook Packages: Springer Protocols

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