Abstract
High-throughput process development is more and more used in chromatography. Limitations are the tools provided by the manufacturers. Here, we describe a method to select ion exchange chromatographic media using a 96-well filter microplate.
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References
Charlton H, Galarza B, Beacon B, LeRiche K, Jones R (2006) Chromatography process development using 96-well microplate formats. BioPharm Int 19:20–27
Bensch M, Schulze Wierling P, von Lieres E, Hubbuch J (2005) High throughput screening of chromatographic phases for rapid process development. Chem Eng Technol 28:1274–1284
Mazza CB, Rege K, Breneman CM, Sukumar N, Dordick JS, Cramer SM (2002) High-throughput screening and quantitative structure-efficacy relationship models of potential displacer molecules for ion-exchange systems. Biotechnol Bioeng 80:60–72
Rege K, Pepsin M, Falcon B, Steele L, Heng M (2006) High-throughput process development for recombinant protein purification. Biotechnol Bioeng 93:618–630
Thiemann J, Jankowski J, Rykl J, Kurzawski S, Pohl T, Wittmann-Lievold B, Schluter H (2004) Principle and applications of the protein-purification-parameter screening system. J Chromatogr A 1043:73–80
Bergander T, Nilsson-Välimaa K, Öberg KM, Lacki K (2008) High-throughput process development: determination of dynamic binding capacity using microtiter filter plates filled with chromatography resin. Biotechnol Prog 24:632–639
Linden T (2001) Untersuchungen zum inneren transport bei der proteinadsorption an poröse medien mittels konfokaler laser-rester-mikroskopie. Heinrich-Heine University, Dusseldorf, p 227
Pezzini J, Joucla G, Gantier R, Toueille M, Lomenech A-M, Le Sénéchal C, Garbay B, Santarelli X, Cabanne C (2011) Antibody capture by mixed-mode chromatography: a comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins. J Chromatogr A 1218:8197–8208
Cabanne C, Santarelli X (2018) Mixed mode chromatography, complex development for large opportunities (Review). Curr Protein Pept Sci 19:1–7
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Cabanne, C., Santarelli, X. (2021). Media Selection in Ion Exchange Chromatography in a Single Microplate. In: Labrou, N.E. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 2178. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0775-6_4
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DOI: https://doi.org/10.1007/978-1-0716-0775-6_4
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0775-6
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