Abstract
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) allows amplification and detection of RNA or DNA rapidly and relatively inexpensively. Here we describe how RT-LAMP can be utilized to detect Zika virus in human urine or serum samples or Aedes mosquito samples. This can be completed in under 30 min and without first isolating the RNA from the sample.
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Acknowledgements
This work was supported by the Maureen and Ronald Hirsch family philanthropic contribution. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of this work.
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Lamb, L.E., Bartolone, S.N., Chancellor, M.B. (2020). Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP). In: Kobinger, G., Racine, T. (eds) Zika Virus. Methods in Molecular Biology, vol 2142. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0581-3_12
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DOI: https://doi.org/10.1007/978-1-0716-0581-3_12
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