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Immunomodulatory Effect of Fasciola hepatica Excretory–Secretory Products on Macrophages

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Fasciola hepatica

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2137))

Abstract

The liver fluke, Fasciola hepatica, infects a wide range of mammals including humans and leads to chronic disease. Like other helminths, F. hepatica migrates and survives in the host tissues after penetrating the intestinal wall to enter the peritoneal cavity, and then migrates through the liver before finally inhabiting the bile ducts. To avoid the antihelminthic immune response during migration, F. hepatica releases excretory–secretory products (FhESP) that exert various immunomodulatory effects, such as alternative macrophage activation or programmed cell death induction. Here, we describe the currently available techniques for studying macrophage activation and apoptotic cell death triggered by purified FhESP originating from the adult F. hepatica fluke.

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Acknowledgments

This work was supported by SECyT-UNC No: 30720150100933CB, FONCyT PICT No 2015-1425 and CONICET, PIP No: GI 112201 501002 60. We would like to thank Dr. Paul Hobson, a native speaker, for revision of the manuscript.

Author information

Authors and Affiliations

  1. Laboratorio de Parasitología y Micología, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Haya de la Torre y Medina Allende, Ciudad Universitaria, Córdoba, Argentina

    Lorena Guasconi, Marianela C. Serradell, Diana T. Masih & Laura S. Chiapello

  2. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET. Haya de la Torre y Medina Allende, Ciudad Universitaria, Córdoba, Argentina

    Lorena Guasconi & Laura S. Chiapello

  3. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas (CIDIE), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC), Córdoba, Argentina

    Marianela C. Serradell

Authors
  1. Lorena Guasconi
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  2. Marianela C. Serradell
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  3. Diana T. Masih
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  4. Laura S. Chiapello
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Corresponding authors

Correspondence to Diana T. Masih or Laura S. Chiapello .

Editor information

Editors and Affiliations

  1. Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil

    Martin Cancela

  2. Unidad de Biología Parasitaria, Instituto de Higiene Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay

    Gabriela Maggioli

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Guasconi, L., Serradell, M.C., Masih, D.T., Chiapello, L.S. (2020). Immunomodulatory Effect of Fasciola hepatica Excretory–Secretory Products on Macrophages. In: Cancela, M., Maggioli, G. (eds) Fasciola hepatica. Methods in Molecular Biology, vol 2137. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0475-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0475-5_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0474-8

  • Online ISBN: 978-1-0716-0475-5

  • eBook Packages: Springer Protocols

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