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Imaging and Electrophysiology of Individual Neurites Functionally Isolated in Microchannels

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Neurohistology and Imaging Techniques

Part of the book series: Neuromethods ((NM,volume 153))

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Abstract

The present chapter describes fabrication and utilization of neurite-isolation microelectrode arrays (NI-MEAs). The neurite-isolation device makes it possible to functionally isolate individual neurites from neuronal cell bodies as the neurites are forced to grow into narrow microchannels. Being microfluidic setups, the NI devices can be conveniently employed to carry out laser axotomy, study retrograde/anterograde transport in single axons, investigate the mutual effects of soluble factors (e.g., amyloid-β peptides) secreted by different neuronal populations (each “housed” in a different macrochannel), or to create the so-called axonal diodes imposing unidirectional axonal connectivity, to name but a few options. A separately made MEA is assembled with the NI device so that each of its microchannels is connected to one microelectrode. Unlike in patch-clamp, electrophysiology performed with the aid of NI-MEA is “massively parallel” as many neurites can be recorded simultaneously, and the data acquisition is noninvasive as the electrodes are not forced into a physical contact with the neurites. The capability of such setup is demonstrated here by recording the effects of different concentrations of nerve growth factor (NGF) on the electrical activity of individual neurites of superior cervical ganglions in primary culture, during their outgrowth into the microchannels. Simultaneous microscopic (e.g., immunofluorescence) observation of multiple neurites and their somata is an inherent extra option permitting to correlate their electrical activity with other physiological parameters, recorded by optical means.

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Abbreviations

AID:

Axon isolation device

Ig:

Immunoglobulin

iPSC:

Induced pluripotent stem cells

MEA:

Micro/Multielectrode array

NGF:

Nerve growth factor

NI-MEA:

Neurite-isolation MEA

PDMS:

Polydimethylsiloxane

PBS:

Phosphate buffered saline

SCG:

Superior cervical ganglion

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Authors and Affiliations

  1. Institute of Solid State Electronics, TU Wien, Vienna, Austria

    Heinz D. Wanzenboeck & Johann K. Mika

  2. Department of Pathobiology of the Nervous System, Center fro Brain Research, Medical University of Vienna, Vienna, Austria

    Petra Scholze

Authors
  1. Heinz D. Wanzenboeck
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  2. Petra Scholze
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  3. Johann K. Mika
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Corresponding author

Correspondence to Heinz D. Wanzenboeck .

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Editors and Affiliations

  1. Czech Academy of Sciences and Charles University, Prague, Czech Republic

    Radek Pelc

  2. Department of Psychiatry, University of Saskatchewan, Saskatoon, SK, Canada

    Wolfgang Walz

  3. Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, SK, Canada

    J. Ronald Doucette

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Wanzenboeck, H.D., Scholze, P., Mika, J.K. (2020). Imaging and Electrophysiology of Individual Neurites Functionally Isolated in Microchannels. In: Pelc, R., Walz, W., Doucette, J.R. (eds) Neurohistology and Imaging Techniques. Neuromethods, vol 153. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0428-1_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0428-1_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0426-7

  • Online ISBN: 978-1-0716-0428-1

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