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Standardizing Chondrocyte Isolation and Articular Cartilage Decellularization: A Versatile Bioink for Tissue Engineering Applications

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Methods in Molecular Biology

Part of the book series: Methods in Molecular Biology

Abstract

The extracellular matrix (ECM) is a noncellular component of tissues that provides structural and biochemical support to cells. The purpose of decellularization is to provide a tissue-specific niche to preserve the architecture, composition, and signaling molecules of the ECM. The current protocol discusses the standardization of chondrocyte isolation and the preparation of acellular ECM as a bioink additive from human native articular cartilage. Isolated chondrocytes with bioink additives provide a tissue-specific microenvironment. Herein, we discuss a standardized protocol with multiple applications in the area of organ-on-a-chip model development, spheroid formation, microfluidics platform, bioprinting, and tissue engineering. Cartilage tissue engineering is complex owing to the heterogeneous complex proteins, which are a challenge to synthesize; hence, this protocol in many ways offers cues to exploit the acellular ECM for multiple ongoing research studies.

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Acknowledgments

The research was funded and supported by the DST SERB POWER Fellowship (File No: SPF/2021/000126) and carried out at the Global Medical Education and Research Foundation.

Author Contributions

  • U. U.: Planning, designing, execution, and standardization of the protocol; interpretation of results; and manuscript writing and review.

  • K.S: Research supervisor.

  • L. K. C.: Concept, result interpretation, project guarantee, manuscript review, and approval.

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Authors and Affiliations

  1. Stem Cell Unit, Global Medical Education and Research Foundation Lakdikapul, Hyderabad, Telangana, India

    Upasna Upadhyay & Lakshmi Kiran Chelluri

  2. Department of Biotechnology, Koneru Lakshmaiah Education Foundation Deemed to be University, Vaddeswaram, Vijayawada, Andhra Pradesh, India

    Upasna Upadhyay & Kamma Srinivasulu

  3. Advanced Diagnostics and Therapeutics, Gleneagles Global Hospitals, Lakdikapul, Hyderabad, Telangana, India

    Lakshmi Kiran Chelluri

Authors
  1. Upasna Upadhyay
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  2. Kamma Srinivasulu
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  3. Lakshmi Kiran Chelluri
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Corresponding author

Correspondence to Lakshmi Kiran Chelluri .

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Upadhyay, U., Srinivasulu, K., Chelluri, L.K. (2024). Standardizing Chondrocyte Isolation and Articular Cartilage Decellularization: A Versatile Bioink for Tissue Engineering Applications. In: Methods in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/7651_2024_534

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  • DOI: https://doi.org/10.1007/7651_2024_534

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  • Publisher Name: Springer, New York, NY

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